EFFECT OF VITAMIN D ADMINISTRATION ON VASCULAR ENDOTHELIAL GROWTH FACTOR EXPRESSION AND ANGIOGENESIS NUMBER IN ORTHODONTIC TOOTH MOVEMENT OF PREGNANT WISTAR RAT

  • Raden Ajeng Anindya Ratri Nareswari Faculty of Dental Medicine, Universitas Airlangga
  • Ida Bagus Narmada Faculty of Dental Medicine, Universitas Airlangga
  • Irwadi Djaharu’ddin Faculty of Dental Medicine, Universitas Airlangga
  • Dwi Rahmawati Faculty of Dental Medicine, Universitas Airlangga
  • Nurul Aisyah Rizky Putranti Faculty of Dental Medicine, Universitas Airlangga
  • Alexander Patera Nugraha Faculty of Dental Medicine, Universitas Airlangga
Keywords: Angiogenesis, Vascular endothelial growth factor, Orthodontic tooth movement, Pregnancy, Vitamin D

Abstract

Objective: To analyze the effect of vitamin D administration on vascular endothelial growth factor (VGEF) expression and angiogenesis number during orthodontic tooth movement (OTM) in pregnant Wistar rats (Rattus norvegicus). Methodology: This was an experimental study with post-test only control group design. Lameshow’s sample size formula was used with random sampling method. Twenty eight female Wistar rats were divided into 2 control groups (C+7 and C+14) and 2 experimental groups (E+7 and E+14). Their pregnancy was synchronized, 30g/mm force closed coil spring was given between their incisors and 1st left maxillary molar. Vitamin D (0.2mg/kg) was given every 3 days to E+7 and E+14. Maxillary bone of all groups was extracted. Angiogenesis number was examined by means of Hematoxylin Eosin staining while VEGF expression was examined with Immunohistochemistry staining. Mann-Whitney and analysis of variance with Tukey HSD post-hoc test (p <0.05) was performed based on Levene’s test and Kolmogorov-Smirnov test result (p >0.05). Results: The lowest angiogenesis number was found in C+7 (11.11 ±5.95) and the highest was found in E+14 (23.83 ±7.10). Significant difference was obtained between C+7 and E+14 (p =0.003, p <0.05); E+7 and E+14 (p =0.013, p <0.05). The lowest VEGF expression was found in E+14 (3.09 ±1.14) while the highest was found in C+14 (6.26 ±2.39). There was no significant difference between each group (p >0.05). Conclusion: Vitamin D administration did not significantly increase VEGF expression and angiogenesis number in OTM of pregnant rats.

Author Biographies

Ida Bagus Narmada, Faculty of Dental Medicine, Universitas Airlangga
Orthodontics Department
Irwadi Djaharu’ddin, Faculty of Dental Medicine, Universitas Airlangga
Orthodontics Department
Dwi Rahmawati, Faculty of Dental Medicine, Universitas Airlangga
Orthodontics Department
Nurul Aisyah Rizky Putranti, Faculty of Dental Medicine, Universitas Airlangga
Orthodontics Department
Alexander Patera Nugraha, Faculty of Dental Medicine, Universitas Airlangga
Orthodontics Department
Published
2019-11-06
Section
Original Article